The Deal Lab
Mercer University - Biology Department
Mercer University - Biology Department
Using the fruit fly to understand sleep.
What causes sleep decline as we age?
Sleep is a biological need for all animals that can greatly effect psychological and physical health. However, as we age not only do we become more susceptible to disease, but our sleep also suffers. We have trouble falling asleep, and we wake up more often at night. We want to understand what are the major molecular and genetic contributors that effect age-related sleep decline.
How do mitochondria contribute to sleep function?
Mitochondria are master regulators of metabolism. They not only supply most of our cell's energy needs, but they can also be critical for lipid homeostasis, Calcium signaling, and cell death. Recent research has suggested that mitochondria may be a key regulator in sleep regulation. We want to investigate mitochondria and sleep in multiple ways. Check out the Research page for more details.
from iNaturalist by Alexis Orion
We use the fruit fly as a model to study sleep and sleep dysfunction. With a great many neurological and genetic tools available, the fruit fly is a great model for studying neurobiology. It is also incredibly convenient for aging experiments since they only live for about three months.
While we use many tools, some of the key tools we use are:
Drosophila Activity Monitor
This system allows us to load individual flies into a monitor and track their activity and sleep over 24 hours. Combined with genetic manipulation like RNA interference, this can be a powerful system.
Fluorescent Reporters
By dissecting and imaging the brains of fruit flies, we can use these reporters to see if there is altered mitochondrial structure or health (e.g. reactive oxygen species or mitochondrial turnover).